Guner, A.; Kibarer, G. (2001). The flattening of the curves at higher molecular weight is in agreement with an increased branching density. what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. Colloid Polym. J Control Release. Correspondence to 2011;16(2):518. Because of the recent animal protection rules which have restricted animal experiments [19], we used human cadaver skin as an alternative to animals. 2010;4(1):117. (2000). Federal government websites often end in .gov or .mil. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. Journal of Physical Chemistry, 58(11), 953-957. Search 2011;5(2):95132. 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. Advances in Physical Chemistry, Vol. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Fixable, Sulen und Kartuschen fr die Chromatographie, Kunststoffartikel und Zubehr fr das Labor, Spektroskopie, Element- und Isotopenanalyse, Alle Themen fr Hilfe und Support anzeigen, Status und Nachverfolgung von Bestellungen, Reagenzien und Kits fr die Neurowissenschaft, Discover how to track and trace cells by imaging , Hier finden Sie unsere brigen Tracking-Produkte fr Sugetier-Zellkulturen, Molecular Probes Dextran-Konjugate Tabelle14.4. Hydrodynamic radii ( RH) were determined by dynamic light scattering. European Polymer Journal, 37, 619-622. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. (2013). Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. Stenekes, R.J.H. Skin barrier disruption by acetone: observations in a hairless mouse skin model. TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. Walkenhorst, S.; Olivier, R. (2002). All branched polysaccharide types could be described approximately by the hyperbranching theory. Keywords: FITC dextran; intestinal permeability; transit time. NCI CPTC Antibody Characterization Program. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear -(1-6)-linked glucose chain with however a low percentage (2-5%) of -(1-3) branches distributed along the chain4. Whiteside PJD, Chininis JA, Schellenberg MW, Qian C, Hunt HK. ; Fran, H.P. The molecular weight of dextran samples can be obtained by a variety of methods including light scattering, HPLC/SEC, sedimentation, osmometry and end-group analysis. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. If there are particles in the sample, the incident laser light gets scattered in all directions. Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. (1937). Hi Alan F Rawle sir, Can you help me in calculating the size of dextran 6 (molecular weight= 6000) and dextran 70 (molecular weight 70000) Porcine wound models for skin substitution and burn treatment. Journal of Chemical Education, 49(8), 577-579. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. Microporation applications for enhancing drug delivery. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. ACS Nano. A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. Elias, Hans-Georg (1975). The Viscosity of Dilute Solutions of Long-Chain Molecules. 2004;25(9):155967. Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. 2005;22(4):5505. (2012). The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. 151-166. Drug Deliv Syst. Polymolecularity and polydispersity in Molecular weight determinations. In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). Journal of Controlled Release, 119, 5-24. Before The Transdermal diffusion cell system was protected from light. Increased epidermal laser fluence through simultaneous ultrasonic microporation. There is a deviation from linearity after 580kDa [69, 70]. HSE and DK helped to porate the skin and to measure TEWL. The range of temperatures used was 25-50 (0.1) C and it was regulated by thermostatic bath Haake C. The viscosity measurements required Hagenbach-Couette correction time (tg 37.31s). WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Google Scholar. FITC-dextran solution (600L) was added to the donor chamber. Kirkland, J. J.; Dilks, C. H.; Rementer, S. W. (1992). Chem. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection Doke SK, Dhawale SC. (2003). Dextrans in Aqueous Solution. International Journal of Pharmaceutics, 277, 99-104. Formul. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. Chem. (1954). Monolayers and Thin Films of Dextran Hydrophobically Modified. Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). The Mark-Houwink exponent bears the signature of a three-dimensional configuration of a polymer chain in the solvent environment. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. 2003;10:33847. Dextrans can function efficiently as anterograde or retrograde tracers, The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. The solution was measured using an Anton Paar DMA35N densitometer. California Privacy Statement, In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. Schematic illustration of transdermal delivery after the application of the microporation device. Table 2 compares the properties of nanoparticles coated with one or other polymer. HHS Vulnerability Disclosure, Help (2003). A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth Advances in Carbohydrate Analysis, 1, 63-144. The effect of branching is best recognized from the molecular weight dependencies of []. Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), 7 8 The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. Polyelectrolytes as secondary calibration standards for aqueous SEC. (1999). The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. ; Rani, K.Y. A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. 20K groups (n=3, *: p<0.05, **: p<0.01). Advances in Cosmetic Surgery. Epub 2012 May 28. B 113, 8020-8025. Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. Drug deliv. Google Scholar. WebDextran produced from Weissella cibaria JAG8 was purified and characterized. Trop J Pharm Res. Smith, David R.; Raymonda, John W. (1972). Ioan, C.E. (2008). These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. Differences were considered statistically significant at p <0.05. The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. FOIA For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. Hydrodynamic radii were measured using Dynamic Light Scattering (DLS) in a Malvern Zetasizer NanoZS DLS (Malvern Instruments, Malvern, UK) at high scattering angle (173) to minimise the effects of dust and supramolecular contamination (NIBS, 173). The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. This is because the macromolecule hydrodynamic radius changes the solution type and temperature via change in their chain exibility. 2. Carbohydrate Polymers 99, 199-207. The hydrodynamic radius is important in predicting transretinal penetration Carroll PJ. Kloster, C.; Bica, C.; Lartigue, C.; Rochas, C.; Samios, D.; Geissler E. (1998). Kim J, Jang JH, Lee JH, Choi JK, Park WR, Bae IH, Bae J, Park JW. dextran hydrodynamic radius. Harding, Stephen E. (2005). It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Macromolecules. 2019;9(3-S):9726. Carbohydrate Polymers, 74, 372-378. where c is the solute concentration (g/cm3), [] is the intrinsic viscosity (cm3/g), and kH represents Huggins constants (cm3/g). High Manufacturing Standards of Molecular Probes Dextrans. (2001). The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was Viscosity of Liquids. Just in case anyone wants to use a different molecular weight to estimate the hydrodynamic size: the calculator is integrated into the classic Zeta When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. Beer, M.U. ; Prasad, D.H.L. Vemulapalli V, Bai Y, Kalluri H, Herwadkar A, Kim H, Davis SP, Friden PM, Banga AK. Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules. Arch Dermatol Res. These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. The human skins were from the back or thigh of a 24-year-old woman. (1987). Pathan IB, Setty CM. European Polymer Journal, 43, 1744-1753. The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). Polysaccharide hydrogels for modified release formulations. Data File Dextran. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. This result could reflect the presence of some aggregated species within the lysozyme sample solution. Not for use in diagnostic procedures. Format), Citation-(EndNote Ed., 9(2), 85-90. Eng. Each microporated sample was incubated in the skin culture medium. Banga AK. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. ; van Nostrum, C.F. Colloid and Polymer Science, 286, 1223-1231. 2015;23(3):2239. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. Biomaterials. The micropores of the human cadaver skin were regenerated according to the time. 2012;27(3):20212. 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861. The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). Progr. Calculation of MarkHouwinkSakurada (MHS) equation viscometric constants for chitosan in any solventtemperatura system using experimental reported viscometric constants data. Chem. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). Figure5 shows the cumulative amount of the FITC-dextran (M.W. A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. Human cadaver skins (fresh human full-thickness skin disc, 12~20mm) were supplied by Biopredic International (Saint-Grgoire, France). Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. 1994, 95-0355. J Cosmet Laser Ther. Keywords: Carbohydrate Polymers, 38, 267-271. Thus, it is a common practice to express the frictional coefficient of rigid structures as. For a values from 0-0.5 a rigid sphere is predicted in a ideal solvent, from 0.5-0.8 a random coil in good solvent, and from 0.8-2 a rigid or rod like shape is expected (stiff chain). Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. 8600 Rockville Pike However, in vitro investigations are very few. Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. In the case of the work performed in references [34, 35, 36] they studied using the number average molecular weight, which rearranges the value of a and forces it to enter into unbranched or linear molecules, on the other hand, if molecular weights are used the value would be less than 0.5. The slope is a value and intercept is equal to the ln kMH value [47].
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